CENPF Antibody

SKU: orb1247678

Description

CENPF Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the predicted size of approx. 358kDa according to NP_057427. Therefore we cannot recommend an optimal concentration.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: GLESKGSENCKVQ
• Target: CENPF
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CENPF, centromere protein F (350/400kD, mitosin), CENF, PRO1779, mitosin, AH antigen, CENP-F kinetochore protein, centromere protein F (400kD), cell-cycle-dependent 350K nuclear protein, centromere protein F, 350/400ka (mitosin), RP11-262H5.1, hcp-1, centromere protein F, mitosin

orb1247678 (3.75 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CENPF

UniProt

P49454

RefSeq:AAA86889.1, AAA82935.1, AAA82889.1