Rabbit CENP-F/Mitosin Antibody

SKU: orb1528072

Description

CENP-F/Mitosin Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 2275 and 2325
• Target: CENP-F/Mitosin
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS

Detection of human CENP-F/Mitosin by immunohistochemistry. Sample: FFPE section of human ewing sarcoma. Antibody: Affinity purified rabbit anti-CENP-F/Mitosin used at a dilution of 1:200 (1µg/ml).

Detection of human CENP-F/Mitosin by WB and IP.Samples: HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-CENP-F/Mitosin antibody orb1528072 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.

Quick Database Links

Gene Symbol

CENP-F/Mitosin

UniProt

P49454

RefSeq (Protein):NP_057427.3