CENP-Q antibody

• Catalog Number: orb344648
• Category: Antibodies
• Description: CENP-Q antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, Multiplex Assay, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length human CENP-Q recombinant protein.
• Concentration: 1.15 mg/mL
• Dilution range: ELISA: 1:5,000 1:20,000, IF: User Optimized, WB: 1:100 - 1:500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human CENP-Q protein. A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (71%), mouse (65%) and rat (60%). Cross-reactivity with CENP-Q from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q7L2Z9
• NCBI: 40068061
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-CENP-Q Antibody, CenpQ, centromere pro
• Note: For research use only
• Application notes: This protein A purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26-31 kDa in size corresponding to human CENP-Q by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa whole cell lysate using CENP-Q antibody

Western blot analysis of HeLa whole cell lysate using CENP-Q antibody