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Catalog Number | orb1252180 |
---|---|
Category | Antibodies |
Description | CELA3B Antibody [CELA3B/1257] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CELA3B/1257 |
Tested applications | ELISA, FC, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG1 |
Immunogen | A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody. |
Antibody Type | Primary Antibody |
Concentration | 0.2 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CELA3B |
UniProt ID | P08861 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Chymotrypsin-like elastase family member 3B, Elast Read more... |
Note | For research use only |
Application notes | ELISA: 2-4 ug/ml; order BSA free format for coatingFlow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlIHC-P: 1-2 ug/ml for 30 min at RTOptimal dilution of the Elastase 3B antibody should be determined by the researcher.1. FFPE staining requires |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Elastase 3B Antibody (clone CELA3B/1257). Confirmation of Integrity and Purity of the Antibody.