CELA3B Antibody / Elastase 3B

• Catalog Number: orb750072
• Category: Antibodies
• Description: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
• UniProt ID: P08861
• Tested applications: ELISA, IHC-P, WB
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Elastase 3B antibody should be determined by the researcher.1. FFPE staining requires
• Antibody Type: Primary Antibody
• Clone Number: CELA3B/1257
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Elastase 3B antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE mouse pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free Elastase 3B Antibody (clone CELA3B/1257). Confirmation of Integrity and Purity of the Antibody.

Western blot of human PANC-1 cell lysate using Elastase 3B antibody (CELA3B/1257). Predicted molecular weight ~29 kDa.