CEBPZ Antibody

SKU: orb1823922

Description

C/EBPZ (CCAAT/enhancer-binding protein Z), also known as CBF, CBF2, NOC1 or HSP-CBF is a 1,054 amino acid nuclear protein belonging to the CBF/MAK21 family. C/EBPZ stimulates transcription from the HSP70 and HSP40 promoters in a NF-Y dependent manner, requiring an intact NF-Y trimer which binds to the DNA. C/EBPZ is thought to be a potential tumor suppressor gene, and aberrant methylation of the C/EBPZ promoter may be involved with acute myeloid leukemia. The C/EBPZ gene is conserved in a wide number of species including chimpanzee, canine, bovine, mouse, rat, chicken, zebrafish and C. elegans and is located on human chromosome 2. Chromosome 2, the second largest human chromosome, consists of 237 million bases encoding over 1,400 genes, comprising approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2.

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2c
• Clone No.: PCRP-CEBPZ-2D8
• Immunogen: The protein domain of CEBPZ protein was used as the immunogen for the CEBPZ antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using CEBPZ Mouse Monoclonal (PCRP-CEBPZ-2D8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

Flow cytometry testing of PFA-fixed human HeLa cells with CEBPZ antibody (clone PCRP-CEBPZ-2D8) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

SDS-PAGE analysis of purified, BSA-free CEBPZ antibody (clone PCRP-CEBPZ-2D8) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q03701