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Item 1 of 16
Item 1 of 16
DDIT3 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb2563499
| Catalog Number | orb2563499 |
|---|---|
| Category | Antibodies |
| Description | DDIT3 Recombinant Rabbit Monoclonal Antibody |
| Target | DDIT3 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Purification | Affinity purified by Protein A |
| Immunogen | A synthesized peptide derived from human DDIT3 (140-169aa) |
| UniProt ID | P35638 |
| MW | 19 kDa |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:1000-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Antibody Type | Primary Antibody |
| Clone Number | 6C8 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | GADD153; CHOP; Growth arrest and DNA damage-induci Read more... |
| Research Area | Cell Signaling, Diabetes Research, Intercellular S Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
25 ug total protein per lane of various lysates probed with DDIT3 monoclonal antibody, unconjugated (orb2563499) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with DDIT3 Monoclonal Antibody, Unconjugated (orb2563499) at 1:100overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
