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CDKN1B/p27 Kip1 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IHC, IHC-P, WB |
|---|---|
| Dilution Range | IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.1 - 0.3 µg/ml) |
| Reactivity | Human |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence C-KRPATDDSSTQNK, from the internal region of the protein sequence according to NP_004055.1. |
| Target | CDKN1B / p27 Kip1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
| Concentration | 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Antibody (0.1 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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CDKN1B/p27 Kip1 Antibody (orb1538206)
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