CDKN1B/p27 KIP 1 Rabbit Polyclonal Antibody

SKU: orb11204

Description

CDKN1B/p27 KIP 1 Rabbit Polyclonal Antibody

Research Area

CIP/KIP Family, CIP/KIP family, Cell Cycle, Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Gallus, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P27 kip1 (101-198/198aa)
• Target: CDKN1B
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p27 KIP 1; CDN1B_HUMAN; AA408329; AI843786; Cdki1b; CDKN 1B; CDKN 4; CDKN1B; CDKN1B; CDKN4; CDKN4; Cyclin Dependent Kinase Inhibitor 1B; Cyclin Dependent Kinase Inhibitor 1B; Cyclin dependent kinase inhibitor p27; Cyclin dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B(p27, Kip1); Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor p27 Kip1; KIP 1; KIP1; MEN1B; MEN4; OTTHUMP00000195098; OTTHUMP00000195099; p27; p27 Kip1; P27-like cyclin-dependent kinase inhibitor; P27KIP1.

Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

CDKN1B (p27Kip1) was immunoprecipitated from mouse kidney tissue with orb11204 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Goat anti-Rabbit IgG (Heavy Chain specific) was used as secondary antibody at 1:5000 dilution. Lane 1: mouse kidney tissue lysate 10 µg (Input). Lane 2: orb11204 IP in mouse kidney tissue lysate. Lane 3: native rabbit IgG IP in mouse kidney tissue lysate (negative control). Secondary, All lanes Goat anti-Rabbit IgG (Heavy Chain specific), HRP Conjugated, 1:5000.

Sample: 293T (Human) Cell Lysate at 30 ug, U2os (Human) Cell Lysate at 30 ug, Primary: Anti-CDKN1B'p27 KIP 1 (orb11204) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.

Quick Database Links

Gene Symbol

CDKN1B

UniProt

P46527