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CDKAL1 Antibody

SKU: orb1249596

Description

CDKAL1 Antibody

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA, WB
Predicted ReactivityCanine, Human, Mouse, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:This antibody has been described in a publication and it has been successfully used on Human islets and pancreatic insulinoma INS-1 and MIN6 cells . Preliminary experiments gave an approx. 39kDa band in Human Brain (Cerebellum) lysates after 0.5µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.1kDa according to NP_060244.2. The 39kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-QVKKQRTKDLSR
TargetCDKAL1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CDK5 regulatory subunit associated protein 1-like 1 , FLJ20342, FLJ46705, MGC75469

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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