CDKAL1 Antibody

SKU: orb666245

Description

Rabbit polyclonal antibody to CDKAL1

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human CDKAL1. The exact sequence is proprietary.
• Target: CDKAL1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Anti-Threonylcarbamoyladenosine tRNA methylthiotransferase antibody, anti-CDK5 regulatory subunit-associated protein 1-like 1 antibody, anti-tRNA-t(6)A37 methylthiotransferase antibody

Western blot analysis of CDKAL1 expression in HEK293T (A), HepG2 (B), mouse kidney (C) whole cell lysates. (Predicted band size: 11; 54; 65 kD; Observed band size: 55-65 kD)

Immunofluorescent analysis of CDKAL1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

CDKAL1

UniProt

Q91WE6, Q5VV42

NCBI Gene ID

68916, 54901