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CDH1/E Cadherin Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-P, IP, WB |
|---|---|
| Dilution range | Flo (1:100 - 1:200), ICC (1:100 - 1:500), IF (1:100 - 1:500), IHC-P (6 µg/ml), IP (1:10 - 1:100), WB (1:500 - 1:1000) |
| Reactivity | Bovine, Canine, Human, Monkey, Porcine, Rabbit, Rat, Zebrafish |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human E Cadherin. |
| Target | CDH1 / E Cadherin |
| Purification | Immunoaffinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol. PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Cadherin 1 Antibody / CDH1 / E-Cadherin [orb1151543]
IHC-P
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Mouse
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100 μg, 20 μgCDH1/E Cadherin Antibody [orb1537044]
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Human, Mouse, Rat
Rabbit
Polyclonal
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50 μlCDH1 / E-cadherin / CD324 Reference Antibody [orb1805855]
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1 mg, 100 μg

Quality Guarantee
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Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemical analysis of E Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of E Cadherin staining in PC12 cells.

Western blot analysis of E Cadherin expression in HEK293T (A); PC12 (B); A431 (C); MCF7 (D); C2C12 (E) whole cell lysates.

Immunoprecipitation of E Cadherin from 0.5mg HEK293F whole cell extract lysate using 5ug of Anti-E Cadherin Antibody and 50ul of protein G magnetic beads (+).
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Protocol Information
CDH1/E Cadherin Antibody (orb1537167)
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