E-Cadherin Antibody / CDH1

• Catalog Number: orb1151543
• Category: Antibodies
• Description: Mouse monoclonal antibody to E-Cadherin
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Purified His-tagged CDH1/Cadherin 1 protein was used as the immunogen for the Cadherin 1 antibody.
• UniProt ID: P12830
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the Cadherin 1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CDH1/4585
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the Cadherin 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This Cadherin 1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human skin with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human skin with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with Cadherin 1 antibody (clone CDH1/4585). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Cadherin 1 antibody (clone CDH1/4585). These results demonstrate the foremost specificity of the CDH1/4585 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Cadherin 1 antibody (clone CDH1/4585) as confirmation of integrity and purity.