CDC25A (S124) Antibody

SKU: orb1925364

Description

Research Area

Cancer Biology, Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	IHC-P, WB
Dilution Range	IHC-P - 1:100, WB - 1:1000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	59087 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

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All lanes: Anti-CDC25A (S124) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-CDC25A (S124) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MCF-7 whole cell lysate. Lane 3: A431 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: Rat testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P30304

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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