Phospho-CDC25A(S124) Antibody

SKU: orb1931290

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:500, IHC-P - 1:100
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB07634
• Immunogen: This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human CDC25A.
• Target: CDC25A
• Molecular Weight: 59087 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

WB analysis of orb1931290.

Immunohistochemical analysis on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-phospho CDC25A S124 Pab is used in Western blot to detect CDC25A in mouse liver tissue lysate

Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1 h, using 459085101 (upper) or Beta-actin (lower).

Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1 h, using 459088101 (upper) or Beta-actin (lower).

Quick Database Links

Gene Symbol

CDC25A

UniProt

P30304

RefSeq (Protein):NP_001780.2, NP_963861.1