CDC25A Antibody

SKU: orb1253771

Description

CDC25A Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: cdc25A (Phospho-Ser76) antibody was raised against a peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.
• Target: CDC25A
• Molecular Weight: 70 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CDC25A2, MEK1, PRKMK1, CDC25M3, MPIP1

Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF-α and CA using cdc25A (Phospho-Ser76).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76).

Quick Database Links

Gene Symbol

CDC25A

UniProt

P30304

RefSeq (Protein):NP_001780.2