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CD97 Antibody

Catalog Number: orb344630

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb344630
CategoryAntibodies
DescriptionCD97 antibody
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Primate
Form/AppearanceLyophilized
Concentration5.0 mg/mL
Buffer/Preservatives0.01% (w/v) Sodium Azide
PurityThis Protein A purified antibody is directed against the mouse CD97 protein. The product was protein A purified from monospecific antiserum. A BLAST analysis was used to suggest cross reactivity with CD97 proteins from mouse (100%) and rat (74%). Only 49% homology is noted for the human homologue. Reactivity against CD97 from other sources is not known.
ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
UniProt IDQ9Z0M6
Tested applicationsELISA, IF, WB
Dilution rangeELISA: 1:10,000 - 1:50,000, IF: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:5,000
Application notesThis Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.
Antibody TypePrimary Antibody
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Alternative namesrabbit anti-CD97 Antibody, CD97 antigen antibody,
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NoteFor research use only
NCBI251823835
CD97 Antibody

Immunofluorescence Microscopy using Biorbyt's Protein A purified anti-CD97 antibody shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:2500 dilution of the primary antibody was used.

CD97 Antibody

Western blot using Biorbyt's Protein A purified anti-CD97 antibody Lane 1: lysate from cells transfected with control DNA. Lane 2: bone marrow lysates taken from CD97 knockout mice. Lane 3: lysate from COS cells expressing Fc-CD97- (5EGF). Load: 10 µl per lane. Primary Antibody: 1:1000 dilution of the primary antibody was used. Secondary Antibody: Exposure: 10-sec. Results: The formation of the CD97 complex is currently under investigation. A shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. No staining was noted from bone marrow lysates taken from CD97 knockout mice. ~65 kDa appearing in all lanes is not known.

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