CD97 Antibody

• Catalog Number: orb344630
• Category: Antibodies
• Description: CD97 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Primate
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
• Antibody Type: Primary Antibody
• Concentration: 5.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IF: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This Protein A purified antibody is directed against the mouse CD97 protein. The product was protein A purified from monospecific antiserum. A BLAST analysis was used to suggest cross reactivity with CD97 proteins from mouse (100%) and rat (74%). Only 49% homology is noted for the human homologue. Reactivity against CD97 from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q9Z0M6
• NCBI: 251823835
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-CD97 Antibody, CD97 antigen antibody,
• Note: For research use only
• Application notes: This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Microscopy using Biorbyt's Protein A purified anti-CD97 antibody shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells. Panel A and C shows similar staining using pre-immune serum. Panel A and B show staining of mock transfected COS cells (no vector). A 1:2500 dilution of the primary antibody was used.

Western blot using Biorbyt's Protein A purified anti-CD97 antibody Lane 1: lysate from cells transfected with control DNA. Lane 2: bone marrow lysates taken from CD97 knockout mice. Lane 3: lysate from COS cells expressing Fc-CD97- (5EGF). Load: 10 µl per lane. Primary Antibody: 1:1000 dilution of the primary antibody was used. Secondary Antibody: Exposure: 10-sec. Results: The formation of the CD97 complex is currently under investigation. A shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. No staining was noted from bone marrow lysates taken from CD97 knockout mice. ~65 kDa appearing in all lanes is not known.