CD97 Antibody (APC)

• Catalog Number: orb1821027
• Category: Antibodies
• Description: CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
• Target: CD97
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: APC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: PHA-activated peripheral blood cells
• UniProt ID: P48960
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: MEM-180
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: TM7LN1, ADGRE5
• Note: For research use only
• Entrez: 976
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD97 (MEM-180) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD97 (MEM-180) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).