CD8b Antibody

SKU: orb2644222

Description

This monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

Images & Validation

• Tested Applications: FACS, Functional Studies, IF
• Dilution Range: Functional Studies (order BSA/azide-free format),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 0.5-1ug/ml
• Reactivity: Mouse
• Application Notes:
  Optimal dilution of the CD8b antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: Rat IgG2b, kappa
• Clone No.: H35-17.2
• Immunogen: Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P10300