CD8A Antibody

• Catalog Number: orb699620
• Category: Antibodies
• Description: This MAb is specific for the 32kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CD8/3892R
• Tested applications: ELISA, FACS
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: Recombinant human full-length protein was used as the immunogen for the recombinant CD8A antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA: order Ab without BSA for coating,Flow cytometry: 1-2ug/million cells
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD8A antibody is available for research use only.
• UniProt ID: P01732
• Storage: Store the recombinant CD8A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD8A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry staining of human lymphocyte-gated PBM cells with recombinant CD8A antibody; Red=recombinant CD8A, Gray= isotype control.