CD8A Antibody

• Catalog Number: orb385672
• Category: Antibodies
• Description: Cluster of Differentiation 8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD4 and 8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C8/1035
• Tested applications: ELISA, FACS
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human CD8a protein was used as immunogen for this anti-CD8 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-CD8 antibody is available for research use only.
• UniProt ID: P01732
• Storage: Store the anti-CD8 antibody (with azide) at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD8 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.