CD8A Antibody

• Catalog Number: orb1261133
• Category: Antibodies
• Description: CD8A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 30kDa
• Target: CD8A
• UniProt ID: P01732
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: T-cell surface glycoprotein CD8 alpha chain, T-lym
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of mouse pancreas, using CD8A antibody (orb1261133) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human tonsil using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CD8A antibody (orb1261133) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using CD8A antibody (orb1261133) at dilution of 1:100. Blue: DAPI for nuclear staining.