CD86 Antibody [PO.3], Rabbit IgG

• Catalog Number: orb1671790
• Category: Antibodies
• Description: CD86 Antibody [PO.3], Rabbit IgG
• Clonality: Recombinant
• Clone Number: PO.3
• Tested applications: Blocking, FA, FC, IHC, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Cd86
• UniProt ID: P42082
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B7-2, PO3, Activation B7-2 antigen, B-lymphocyte a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467). Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of PO.3 (orb1671790) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671790- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.