CD86 Antibody

• Catalog Number: orb749323
• Category: Antibodies
• Description: This antibody recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by Bcell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in Tcell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BU63
• Tested applications: FACS, Functional Studies, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: ARH-77 (B-lymphoblastoid cell line) was used as the immunogen for this CD86 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Functional studies: order BSA/sodium azide-free format,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD86 antibody is available for research use only.
• Entrez: 942
• Storage: Store the CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD86 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human esophageal tumor stained with CD86 antibody (clone BU63).

FACS staining of human PBMCs using CD86 antibody (red, clone BU63) and isotype control (green).

Flow cytometry testing of PFA-fixed human Ramos cells with CD86 antibody (clone BU63); Red = isotype control, Blue = CD86 antibody.

Immunofluorescent staining of PFA-fixed human Ramos cells with CD86 antibody (clone BU63, green) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone BU63) as confirmation of integrity and purity.