CD86 Antibody

• Catalog Number: orb1258868
• Category: Antibodies
• Description: CD86 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein of human CD86.
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 80kDa
• Target: CD86
• UniProt ID: P42081
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CD86 Antibody: CD86 molecule, B70, B7-2, B7.2, LAB
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CD86 antibody (orb1258868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of THP-1 cells using CD86 Polyclonal Antibody (orb1258868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of rat spleen using CD86 Polyclonal Antibody (orb1258868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of mouse spleen using CD86 Polyclonal Antibody (orb1258868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 3 ug CD86 antibody (orb1258868). Western blot was performed from the immunoprecipitate using CD86 antibody (orb1258868) at a dilution of 1:1000.