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CD86 Antibody
SKU: orb1258868
Featured
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein of human CD86. |
| Target | CD86 |
| Molecular Weight | Observed: 80kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CD86 Antibody: CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2
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Western blot analysis of extracts of various cell lines, using CD86 antibody (orb1258868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of THP-1 cells using CD86 Polyclonal Antibody (orb1258868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat spleen using CD86 Polyclonal Antibody (orb1258868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse spleen using CD86 Polyclonal Antibody (orb1258868) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 3 ug CD86 antibody (orb1258868). Western blot was performed from the immunoprecipitate using CD86 antibody (orb1258868) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
CD86
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
IP
Immunoprecipitation
CD86 Antibody (orb1258868)
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