CD86 Antibody

SKU: orb1240001

Description

CD86 Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rabbit
• Application Notes:
  CD86 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: CD86 antibody was raised against a peptide corresponding to 17 amino acids near the center of human CD86.The immunogen is located within amino acids 160 - 210 of CD86.
• Target: CD86
• Molecular Weight: Predicted: 36 kDa Observed: 68 kDa
• Purification: CD86 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: CD86 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD86 Antibody: CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2

Western blot analysis of CD86 in (A) Daudi and (B) Raji cell lysate with CD86 antibody at 1 µg/mL.

Immunohistochemistry of CD86 in human lymph node tissue with CD86 antibody at 2 µg/ml.

Immunofluorescence of CD86 in human lymph node tissue with CD86 antibody at 20 µg/ml. Red: CD86 Antibody (orb1240001) Blue: DAPI staining.

Quick Database Links

Gene Symbol

CD86

UniProt

P42082

RefSeq (Protein):NP_787058