CD86 Antibody

SKU: orb1252802

Description

CD86 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC-P
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLIHC (FFPE): 1-2 µg/mL (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD86 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: BU63
• Immunogen: ARH-77 (B-lymphoblastoid cell line) was used as the immunogen for this CD86 antibody.
• Target: CD86
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B72, FUN-1, CD86, CD86, CD28LG2

IHC testing of human esophageal tumor stained with CD86 antibody (BU63).

FACS staining of human PBMCs using CD86 antibody (BU63).

Quick Database Links

Gene Symbol

CD86

UniProt

P42081