CD80 Antibody [12D9]

• Catalog Number: orb1239277
• Category: Antibodies
• Description: CD80 Antibody [12D9]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 12D9
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: CD80 antibody was raised against the extracellular domain of human CD80.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 32 kDa Observed: 50 kDa
• Target: CD80
• UniProt ID: P33681
• NCBI: NP_005182
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: CD80 Antibody: CD80 molecule, B7, BB1, B7-1, B7.1,
• Note: For research use only
• Application notes: CD80 antibody can be used for detection of CD80 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 μg/ml

Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.

Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL. Red: CD80 Antibody [12D9] (orb1239277) Blue: DAPI staining

Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL. Red: CD80 Antibody [12D9] (orb1239277) Blue: DAPI staining

Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL. Red: CD80 Antibody [12D9] (orb1239277) Blue: DAPI staining

Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.

Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.

Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.