CD8 Antibody (alpha)

SKU: orb2638145

Description

Cluster of Differentiation 8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD4 and 8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FACS
• Dilution Range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD8 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: C8/1035
• Immunogen: Recombinant full-length human CD8a protein was used as immunogen for this anti-CD8 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry staining of lymphocyte-gated human PBM cells with CD8 antibody (clone C8/1035); Gray = unstained, Red = CD8 antibody.

SDS-PAGE Analysis of Purified, BSA-Free Anti-CD8 Antibody (clone C8/1035). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P01732