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Description
Research Area
Immunology & Inflammation
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution Range | ELISA (For coating, order antibody without BSA);Flow Cytometry (2-4ug/million cells in 0.1ml);,Immunofluorescence (2-4ug/ml); Western Blot (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | NT5E/2646 |
| Immunogen | Recombinant full-length human NT5E protein |
| Target | NT5E |
| Source/Expression System | Bioreactor |
| Molecular Weight | 71kDa |
| Purification | Protein G |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. |
| Buffer/Preservatives | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab conjugated to Biotin |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-5&apos antibody, anti-NT antibody, anti-5&apos antibody, anti-nucleotidase (CD73) antibody, anti-5&apos antibody, anti-nucleotidase precursor antibody, anti-5&apos antibody, anti-nucleotidase antibody, anti-ecto antibody, anti-5&apos antibody, anti-nucleotidase antibody, anti-ecto (CD73) antibody, anti-E5NT antibody, anti-Ecto 5&apos antibody, anti-nucleotidase antibody, anti-Ecto-5&apos antibody, anti--nucleotidase antibody, anti-eN antibody, anti-eNT antibody, anti-NT antibody, anti-NT5 antibody, anti-NT5E antibody, anti-NTE antibody, anti-Purine 5 Prime Nucleotidase antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)






