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CD68 Antibody

Catalog Number: orb749341

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749341
CategoryAntibodies
DescriptionThis antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. antibody to CD68 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. CD68 antibody reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. CD68 shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Species/HostMouse
ClonalityMonoclonal
Clone NumberC68/684
Tested applicationsFACS, IF, IHC-P, WB
ReactivityFeline, Human, Mouse, Rat
IsotypeMouse IgG1, kappa
ImmunogenThe subcellular fraction of human alveolar macrophages was used as the immunogen for this CD68 antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis CD68 antibody is available for research use only.
Entrez968
StorageStore the CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
CD68 Antibody

IHC testing of human tonsil (10X) stained with CD68 antibody (C68/684).

CD68 Antibody

Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 antibody.

CD68 Antibody

Immunofluorescent staining of fixed human U-87 MG cells with CD68 antibody (clone C68/684, green) and Reddot nuclear stain (red).

CD68 Antibody

Flow cytometry testing of fixed human U-87 MG cells with CD63 antibody (clone C68/684); Red = isotype control, Blue = CD63 antibody.

CD68 Antibody

Western blot testing of human spleen lysate with CD68 antibody (clone C68/684). Expected molecular weight: 37-110 kDa depending on glycosylation level.

CD68 Antibody

SDS-PAGE analysis of purified, BSA-free CD68 antibody (clone C68/684) as confirmation of integrity and purity.

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