CD68 Antibody

SKU: orb614009

Description

CD68 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000-1:50,000, WB: 1:1000
• Reactivity: Mouse
• Application Notes:
  Anti-CD68 Antibody has been tested in ELISA and Western Blot. Expect a band at ~34.8Da in western blot using appropriate tissues and lysates. Positive control used: mouse heart and kidney in WB.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: CD68 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal region of mouse CD68.
• Target: CD68
• Purity: Anti-CD68 Antibody is directed against mouse CD68 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cd68 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.02 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-CD68 Antibody, Rabbit Anti-Macrosialin Antibody, Gp110, CD68, CD68 Molecule, CD68 Antigen, Scavenger Receptor Class D Member, Macrophage Antigen CD68, SCARD1, LAMP4, Macrosialin

ELISA results of purified Rabbit Anti-CD68 Antibody tested against BSA-conjugated CD68 immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Microwell Blocking Buffer (p/n orb348584), Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and orb348651.

Western Blot of Rabbit Anti-CD68 Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: HeLa + Rec. CD68 (Baculo expressed) protein (10 µg/0.05 µg) [+]. Lane 3: HeLa + Rec. CD68 (Baculo expressed) protein (10 µg/0.02 µg) [+]. Lane 4: HeLa + Rec. CD68 (Baculo expressed) protein (10 µg/0.01 µg) [+]. Lane 5: HeLa whole cell lysate (p/n orb348668) (10 µg) [-]. Primary Antibody: Anti-CD68 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 60 mins at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~60-70kDa recombinant CD68. Observed MW: ~75kDa.

Western Blot of Rabbit Anti-CD68 Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Mouse Liver tissue lysate (p/n orb348718) [+]. Lane 3: Mouse Lung tissue lysate (p/n orb692725) [+]. Lane 4: Mouse Spleen tissue lysate (p/n orb348720) [+]. Lane 5: Mouse Heart tissue lysate (p/n orb348716) [low]. Lane 6: Mouse Testis tissue lysate [low]. Lane 7: Mouse Kidney tissue lysate (p/n orb348717) [low]. Lane 8: Opal Prestained Molecular Weight Marker. Primary Antibody: Anti-CD68 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 60 mins at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~34kDa with glycosylations. Observed MW: ~34kDa.

Quick Database Links

Gene Symbol

CD68

UniProt

P31996

RefSeq (Protein):NP_001277987.1