CD68 Antibody

SKU: orb614008

Description

CD68 antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000-1:50,000, WB: 1:1000
• Reactivity: Mouse
• Application Notes:
  Anti-CD68 Antibody has been tested in ELISA and Western Blot. Expect a band at ~34.8Da in western blot using appropriate tissues and lysates. Positive control used: mouse heart and kidney in WB.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: CD68 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal region of mouse CD68.
• Target: CD68
• Purity: Anti-CD68 Antibody is directed against mouse CD68 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse based on 100% homology for the immunogen sequence. Cross reactivity with Cd68 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.02 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-CD68 Antibody, Rabbit Anti-Macrosialin Antibody, Gp110, CD68, CD68 Molecule, CD68 Antigen, Scavenger Receptor Class D Member, Macrophage Antigen CD68, SCARD1, LAMP4, Macrosialin

ELISA results of purified Rabbit Anti-CD68 Antibody tested against BSA-conjugated CD68 immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Microwell Blocking Buffer (p/n orb348584), Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and orb348651.

Western Blot of Rabbit Anti-CD68 Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: HeLa + Rec. CD68 (Baculo expressed) protein (10 µg/0.05 µg) [+]. Lane 3: HeLa + Rec. CD68 (Baculo expressed) protein (10 µg/0.02 µg) [+]. Lane 4: HeLa + Rec. CD68 (Baculo expressed) protein (10 µg/0.01 µg) [+]. Lane 5: HeLa whole cell lysate (p/n orb348668) (10 µg) [-]. Primary Antibody: Anti-CD68 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 60 mins at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~60-70kDa recombinant CD68. Observed MW: ~75kDa.

Western Blot of Rabbit Anti-CD68 Antibody. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Mouse Liver tissue lysate (p/n orb348718) [+]. Lane 3: Mouse Lung tissue lysate (p/n orb692725) [+]. Lane 4: Mouse Spleen tissue lysate (p/n orb348720) [+]. Lane 5: Mouse Heart tissue lysate (p/n orb348716) [low]. Lane 6: Mouse Testis tissue lysate [low]. Lane 7: Mouse Kidney tissue lysate (p/n orb348717) [low]. Lane 8: Opal Prestained Molecular Weight Marker. Primary Antibody: Anti-CD68 at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n orb347654) at 1:70000 for 60 mins at RT. Block: BlockOut Buffer (p/n orb348644). Expected MW: ~34kDa with glycosylations. Observed MW: ~34kDa.

Quick Database Links

Gene Symbol

CD68

UniProt

P31996

RefSeq (Protein):NP_001277987.1