CD63 (Late Endosomes Marker) Antibody

• Catalog Number: orb1410529
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD63 (Late Endosomes Marker)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LAMP3/4949
• Tested applications: ELISA, FC, Functional Studies, IF, IHC
• Reactivity: Human, Porcine
• Isotype: IgG1
• Immunogen: Full-length human CD63 protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: ELISA; Functional studies; Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)); ,Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 26kDa (core protein); 30-60kDa (glycosylated)
• Target: CD63
• Entrez: 967
• UniProt ID: P08962
• Expression System: Bioreactor
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: gp55; granulophysin; Lysosomal-associated membrane
• Note: For research use only
• Application notes: Positive Control: HL60, THP-1 or NIH/3T3 cells. Human spleen, melanoma or lymphoma.
• Expiration Date: 12 months from date of receipt.