CD63 Antibody

SKU: orb1257586

Description

CD63 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 103-203 of human CD63 (NP_001771.1).
• Target: CD63
• Molecular Weight: Observed: 53kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30

Western blot analysis of extracts of various cell lines, using CD63 Antibody (orb1257586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded rat brain using CD63 antibody (orb1257586) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using CD63 antibody (orb1257586) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using CD63 antibody (orb1257586) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

CD63

UniProt

P08962