CD63 Antibody

SKU: orb1252797

Description

CD63 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLWB: 1-2 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: MX-49.129.5
• Immunogen: Full length human CD63 was used as the immunogen for this antibody.
• Target: CD63
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30

IHC testing of human melanoma stained with CD63 antibody (MX49.129.5).

IHC testing of mouse spleen stained with CD63 antibody (MX49.129.5).

FACS testing of human PBMC: Black = cells alone; Green = isotype control; Red = CD63 antibody PE conjugate.

FACS testing of mouse NIH3T3: Black = cells alone; Green = isotype control; Red = CD63 antibody PE conjugate.

Western blot testing of human spleen lysate with CD63 antibody at 2 ug/ml.

IHC testing of FFPE prostate carcinoma with CD63 antibody.

Immunofluorescence testing of HeLa cells with Alexa Fluor 488 conjugated CD63 antibody (green). F-actin filaments are labeled with Dylight 554 phalloidin (red); nuclei stained with DAPI (blue).

Quick Database Links

Gene Symbol

CD63

UniProt

P08962