CD62p Rabbit Polyclonal Antibody

SKU: orb500998

Description

CD62p Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Cell Biology, Cell Markers, Cell adhesion Molecules, Immunology, Thrombocytic Lineage, Varscular

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse P-selectin (701-768/768aa)
• Target: SELP
• Molecular Weight: 88 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

P-selectin; CD_antigen: CD62P; CD62 antigen-like family member P; GMP 140; GMP-140; GMRP; Granule membrane protein 140; Granulocyte membrane protein; GRMP; LECAM 3; LECAM3; Leukocyte endothelial cell adhesion molecule 3; Leukocyte-endothelial cell adhesion molecule 3; P Selectin; PADGEM; Platelet activation dependent granule-external membrane protein; PSEL; sp-selectin; LYAM3_HUMAN.

Blank control: HUVEC cells (blue). Primary Antibody: Rabbit Anti-CD62p antibody (orb500998), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb500998, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: K562 (Human) Cell Lysate at 30 ug, Huvec (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-CD62p (orb500998) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 140'90 kD, Observed band size: 130 kD.

Tissue/Cell: rat pancreas tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-P-selectin Polyclonal Antibody, Unconjugated (orb500998) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

SELP

UniProt

Q01102

References

Przemysław Kurtyka et al. Biosafety Assessment of a Prototype Centrifugal Pump Rotor for Cardiac Assist System ACS Biomater Sci Eng, (2025)

PubMed: 41478735