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Catalog Number | orb1294259 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to P selectin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human P selectin / CD62P |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:200 |
Conjugation | Unconjugated |
Target | P-selectin | CD62P |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti P-selectin/CD62P antibody at 1/500 dilution, Lysates at 50 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294259 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling P-selectin/CD62P with orb1294259 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Cancer tissue labeling P-selectin/CD62P with orb1294259 at 1/100.
Immunohistochemical analysis of paraffin embedded Human Cancer tissue labeling P-selectin/CD62P with orb1294259 at 1/100.
Immunohistochemical analysis of paraffin embedded human tissue labeling P-selectin/CD62P with orb1294259 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded human tissue labeling P-selectin/CD62P with orb1294259 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 106.1 kDa after removal of the signal peptide.The apparent molecular mass of SELP-hFc is approximately 130-150 kDa due to glycosylation. | |
Mammalian |