CD6 Antibody

• Catalog Number: orb749315
• Category: Antibodies
• Description: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular 'scavenger receptor cysteine-rich' (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. antibody to CD6 can be used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C6/372
• Tested applications: IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human recombinant CD6 protein was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD6 antibody is available for research use only.
• Entrez: 923
• Storage: Store the CD6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human tonsil (10X) stained with CD6 antibody (clone C6/372).

IHC testing of human tonsil (20X) stained with CD6 antibody (clone C6/372).

Immunofluorescent staining of human MOLT4 cells with CD6 antibody (green, clone C6/372) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free CD6 antibody (clone C6/372) as confirmation of integrity and purity.