CD6 Antibody

SKU: orb1252809

Description

CD6 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 3F7B5
• Immunogen: The human rheumatoid synovial T cell line ST-1 was used as the immunogen for this CD6 antibody.
• Target: CD6
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD6, CD6 molecule, T12, TP120, CD6 antigen

IHC testing of human tonsil stained with CD6 antibody (3F7B5).

Formalin/paraffin human tonsil stained with CD6 antibody (3F7B5).

Quick Database Links

Gene Symbol

CD6

UniProt

P30203