CD58 Antibody

SKU: orb1538556

Description

CD58 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:1000)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone No.: 2D11-B10
• Immunogen: CD58 (AAH05930, 1 aa ~ 241 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: CD58
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD58, CD58 antigen, AG3, LFA-3, LFA3, Surface glycoprotein LFA-3, Surface glycoprotein lfa3, CD58 molecule

Anti-CD58 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunofluorescence of monoclonal antibody to CD58 on HeLa cell. [antibody concentration 10 ug/ml]

CD58 monoclonal antibody (M01), clone 2D11-B10 Western blot of CD58 expression in Jurkat.

Detection limit for recombinant GST tagged CD58 is approximately 0.3 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

CD58