CD2 Antibody

SKU: orb749308

Description

CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.

Images & Validation

• Tested Applications: FACS, Functional Studies, IF, WB
• Dilution Range: Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: 1E7E8.G4
• Immunogen: Human thymocytes/Sezary T cells were used as the immunogen for this CD2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human Jurkat cell lysate with CD2 antibody (clone 1E7E8.G4). Expected molecular weight: 38-50 kDa depending on level of glycosylation.

Immunofluorescent staining of human Molt-4 cells with CD2 antibody (clone 1E7E8.G4, green) and Reddot nuclear stain (red).

Flow cytometry staining of human Molt-4 cells with CD2 antibody (clone 1E7E8.G4); Red = isotype control, Blue = CD2 antibody.

SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone 1E7E8.G4) as confirmation of integrity and purity.