CD5 Antibody

• Catalog Number: orb749313
• Category: Antibodies
• Description: This antibody recognizes a 67kDa transmembrane protein, which is identified as CD5. It is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in Tcell areas. CD5 antibody is a pan Tcell marker that also reacts with a range of neoplastic Bcells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large Bcell lymphoma. Aberrant expression is useful in making a diagnosis of mature Tcell neoplasms. CD5 antibody detection is diagnostic in CLL/SLL within a panel of other Bcell markers, especially one that includes anti-CD23. CD5 antibody is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, it can be used in distinguishing thymic carcinoma (+) from thymoma (-). It does not react with granulocytes or monocytes.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CRIS-1
• Tested applications: FACS, Functional Studies, IF, IP
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Stimulated human leukocytes were used as the immunogen for this CD5 antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Functional studies (order BSA/sodium azide-free format),Immunoprecipitation (0.5-1ug/500ug protein lysate)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD5 antibody is available for research use only.
• Entrez: 921
• Storage: Store the CD5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD5 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.