CD47 Antibody (Biotin)

SKU: orb751265

Description

Mouse monoclonal antibody against CD47 conjugated to Biotin

Research Area

Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: Western Blot (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Humanplacenta, brain or ovarian tumors (IHC).

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2c
• Clone No.: CD47/2937
• Immunogen: A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)
• Target: CD47
• Source/Expression System: Bioreactor
• Molecular Weight: ~50kDa
• Purification: Protein A/G
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-Antigenic Surface Determinant Protein OA3 antibody, anti-IAP antibody, anti-Integrin Associated Protein antibody, anti-Integrin Associated Signal Transducer antibody, anti-MER6 antibody, anti-OA3 antibody, anti-Protein MER6 antibody, anti-Rh Related Antigen antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using CD47 Mouse Monoclonal Antibody (CD47/2937) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD47 Mouse Monoclonal Antibody (IAP/2937).

SDS-PAGE Analysis Purified CD47 Mouse Monoclonal Antibody (IAP/2937). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

CD47

UniProt

Q08722

NCBI Gene ID

961