CD47 Antibody (Biotin)

SKU: orb750931

Description

Mouse monoclonal antibody against CD47 conjugated to Biotin

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution Range: Immunofluorescence (2-4ug/ml); Flow Cytometry (5ul per million cells);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: CD47/3019
• Immunogen: A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)
• Target: CD47
• Source/Expression System: Bioreactor
• Molecular Weight: ~50kDa
• Purification: Protein G
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Antigenic Surface Determinant Protein OA3 antibody, anti-IAP antibody, anti-Integrin Associated Protein antibody, anti-Integrin Associated Signal Transducer antibody, anti-MER6 antibody, anti-OA3 antibody, anti-Protein MER6 antibody, anti-Rh Related Antigen antibody

Quick Database Links

Gene Symbol

CD47

UniProt

Q08722

NCBI Gene ID

961