CD47 Antibody

SKU: orb1252798

Description

CD47 Antibody

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: Blocking, FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Functional Activity (Blocking): Order BSA/Azide-free formatFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 min at RTWestern blot: 2-5 µg/mLThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: B6H12.2
• Immunogen: Intact CD47 purified from placenta was used as the immunogen for this antibody.
• Target: CD47
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6

Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35kDa but observed at 35-60kDa depending on glycosylation level.

Quick Database Links

Gene Symbol

CD47

UniProt

Q08722