CD47 Antibody [B6H12.2]

• Catalog Number: orb1252798
• Category: Antibodies
• Description: CD47 Antibody [B6H12.2]
• Target: CD47
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Intact CD47 purified from placenta was used as the immunogen for this antibody.
• UniProt ID: Q08722
• Tested applications: Blocking, FC, IF, IHC, WB
• Application notes: Functional Activity (Blocking): Order BSA/Azide-free formatFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTWestern blot: 2-5 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: B6H12.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Leukocyte surface antigen CD47, Antigenic surface
• Research Area: Cancer Biology, Immunology & Inflammation
• Note: For research use only

Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35kDa but observed at 35-60kDa depending on glycosylation level.