CD44 Antibody (C-term)

SKU: orb1927005

Description

CD44 Antibody (C-term)

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 81538 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LHR, MDU2, MDU3, MIC4

All lanes: Anti-CD44 Antibody at 1:2000 dilution (upper). Lane 1: Hela. Lane 2: Hela-CD44-Knock out. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from Hela, PC-3, HT-1080 cell line (from left to right), using CD44 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

All lanes: Anti-CD44 Antibody (C-term) at 1:1000 dilution. Lane 1: PC-3 whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 90 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P16070