CD44 Antibody (C-term) [Knockout Validated]

• Catalog Number: orb1787823
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CD44 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 688-722 amino acids from the C-terminal region of human CD44.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P16070
• MW: 81538 Da
• Tested applications: WB
• Dilution range: WB: 1:1000, WB: 1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CD44 antigen, CDw44, Epican, Extracellular matrix
• Research Area: Cancer Biology, Immunology & Inflammation, Signal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Hela, PC-3, HT-1080 cell line (from left to right), using CD44 Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

All lanes: Anti-CD44 Antibody at 1:2000 dilution (upper). Lane 1: Hela. Lane 2: Hela-CD44-Knockout. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 82 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.