CD40LG Antibody

• Catalog Number: orb1260644
• Category: Antibodies
• Description: CD40LG Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 79-129 of human CD40LG (NP_000065.1).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:2000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 29kDa
• Target: CD40LG
• UniProt ID: P29965
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-rela
• Note: For research use only
• Application notes: WB: 1:1000 - 1:2000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using CD40LG antibody (orb1260644) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of K561 cells, using CD40LG antibody (orb1260644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of NIH/3T3 cells using CD40LG antibody (orb1260644) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using CD40LG antibody (orb1260644) at dilution of 1:100. Blue: DAPI for nuclear staining.