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Catalog Number | orb607900 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CD40LG/2763 |
Tested applications | ELISA, FC, IF, IHC |
Reactivity | Human |
Isotype | IgG1 |
Immunogen | Recombinant fragment of human CD40LG protein (around aa 108-261) (exact sequence is proprietary) |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Dilution range | ELISA (For coating, order antibody without BSA);Flow Cytometry (1-2ug/million cells);Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined. |
Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | 36kDa |
Target | CD40LG |
Entrez | 959 |
UniProt ID | P29965 |
Expression System | Bioreactor |
Storage | Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
Buffer/Preservatives | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Alternative names | CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T B cell ac Read more... |
Note | For research use only |
Application notes | Positive Control: Jurkat cells. Human tonsil, spleen or thymus tissue (IHC). |
Expiration Date | 12 months from date of receipt. |
ELISA, FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, IF, IHC | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |